ISOLATION OF HERNIARIN FROM MATRICARIA CHAMOMILLA EXTRACTIONS. APPLICATION TO DETERMINE IN CHAMOMILE AND LIPOSOMAL GEL

Abstract

Chamomile (Matricaria chamomilla L.) is a medicinal plant species from the Asteraceae family. It is known to have numerous pharmacological activities, such as hypoglycemic, antistress, analgesic, anti-inflammatory, and antibacterial activities. This study was designed to isolate and quantitative herniarin, a well-known coumarin of chamomile. Herniarin was isolated from the chamomile absolute by vacuum liquid chromatography with n-hexane and chloroform mixed. Structure of herniarin was determined by analyzing the UV-Vis, IR, and NMR spectroscopy data. Herniarin quantitative procedures from chamomile extract and liposomal
gel use HPLC system with DAD detector. This procedure was validated with the specificity, linearity, range, accuracy and precision characteristics.