GENETIC RELATIONSHIPS OF THE ANABAS (ANABAS SP.) USING MITOCHONDRIAL CYTOCHROME OXIDASE SUBUNIT I MARKER GENE

Abstract

Total DNA of the anabas specimens (A1, A2, A3, A4) in Vietnam were extracted using PHUSA-IHHNV kit and according to the process of Phu Sa Biochemical Company. The mitochondrial cytochrome oxidase subunit I (COI) gene were amplified using PCR reaction. The obtained PCR products were checked using 2 % agarose gel electrophoresis and were sequenced with both the forward and reverse primers using an automated sequencer ABI 3730XL of Applied Biosystems by Sangers method. The genetic distances (Dij) between the anabas species in Vietnam (A1, A2, A3, A4) are very small from 0.0046 to 0.0156 and they are all in group 4 with Anabas testudineus in Malaysia so they are the same Anabas testudineus. The researched species of Anabas genus in Vietnam (A1, A2, A3, A4) and some countries in South and Southeast Asia were divided into four groups based on COI marker gene. The groups with far genetic distance include group 2 and group 4 (D24 = 0.1055), group 1 and group 4 (D14 = 0.1041), group 1 and group 3 (D13 = 0.0910). The groups with medium genetic distance include group 2 and group 3
(D23 = 0.0849), group 1 and group 2 (D12 = 0.0772). The groups with near genetic distance include group 3 and group 4 (D34 = 0.0285). The species Anabas testudineus in India and Anabas testudineus in Myanmar should be re-identified Anabas cobojius in India and Anabas cobojius in Myanmar belong to group 1.