EFFECTING OF SUCROSE CONCENTRATIONS AND INOCULUM DENSITY ON ADVENTITIUOS ROOT GROWTH IN CELL SUSPENSION CULTURE OF Panax vietnamensis AND INITIALLY GROWTH IN A BIOREACTOR

Abstract

It has been demonstrated that the difference of initial sucrose concentrations (0, 3, 5, 7, 9 %) effected on adventitious root growth in cell suspension culture of Panax vietnamensis. The maximum adventitious root growth was obtained in a concentration of 5% sucrose. The density of initial explants also effected to the course of growth. The concentration of 30 mM.2% inoculum density was optimal, and further incresase of inoculum density led to a specific decrease of the rate root growth. Adventitious root of Panax vietnamensis developed well in 5 - liter bioreactors containing 2 liters of SH medium (working medium) supplemented
with 5 mg l-1 IBA, and 0,02 mg/l BA. The growth rate of root was 5.6 folds after 45 days in this condition.