EVALUATION OF THE ANTI-INFLAMMATORY AND ANTICANCER ACTIVITIES OF CATECHINS EXTRACTED FROM GREEN TEA (CAMELLIA SINENSIS) IN SOME CANCER CELL LINES

Abstract

Catechins from Green tea (Camellia sinensis) is widely known for many biological activities, special anti-cancer and anti-inflammatory properties. Catechins were extracted by mechanical blending, and using citric acid, with 2.31% in extraction efficiency. The result of determining by HPLC-UV showed that Catechin< EC < EGC < ECG < EGCG in concentration compared to dry powder, reaching a total content of 634.51 mg/g, higher than tea powder (537.65 mg/g). Then, anti-inflammatory and anticancer activities of these extracts were investigated. Cell viability assay (MTT) showed that catechin extracts were not toxic to Normal Human Dermal Fibroblasts Cells. They even exhibited anti-inflammatoryactivities by suppressing the production of NO and proinflammatory cytokines. The results also showed that the extracted catechin powder also showed good anti-inflammatory activity, at a concentration of 45?g/mL, NO proliferation reached 44.4%. Anticancer activities of these extracts were displayed through their ability to inhibit the growth of cancer cell lines: liver cancer cell line (HepG2), lung cancer cell line (A549), leukemia cancer cell line (K562), with IC50 values of 181.1 µg/mL, 186 µg/mL, 137.8 µg/mL, respectively. So catechin extracts from green tea may have the potential to be applied in pharmaceutical.