DETERMINATION OF TOTAL POLYPHENOLS FROM Eclipta prostrata (L.) Hassk. Asteraceae BY FOLIN-CIOCALTEU METHOD

Abstract

Eclipta prostrata (L.) Hassk. Asteraceae is a universal tree found in Vietnam. It was necessary to develop an analytical method to determine the content of total polyphenols (TP) in this herbal drug. The preanalytical method was standardized for analysis time, wavelength, and the standard to use. The optimum conditions were: 45 min; 760 nm; Gallic acid respectively. Under these conditions, validation by UV/Vis spectrophotometry proved to be reliable for TP of the crude extracts (CE) from the dried aerial parts Eclipta prostrata (L.) Hassk. Asteraceae. Standardization is required for every herbal drug, and this method proved
to be linear, precise, accurate, reproducible, robust, and easy to perform. The results showed that the contents of total polyphenols (TP) in some samples of Eclipta prostrata CE are about over 20mg/g extract (calculated on the drying weights)