ISOLATION OF HIPPADINE FROM THE BULBS OF CRINUM LATIFOLIUM L. AMARYLLIDACEAE

Abstract

The purpose of study is extraction and isolation of hippadine from the bulbs of Crinum latifolium for using as a reference standard for quality control of herbs and hippadine-containing preparations. Bulbs powder of Crinum latifolium was percolated with 70% ethanol, percolate obtained was evaporated at 65 0C to give a liquid extract. The extract was fractionated by distribution with n-hexane. The n-hexane extract was then chromatographed on silicagel 60 (0.04 - 0.063 mm). Hippadine was isolated, purified and elucidated by NMR, MS, IR and UV-Vis spectrum. The purity of hippadine was determined by HPLC-PDA. About 565,3 mg of hippadine was isolated and its purity was detemined as pure as 100%. Hippadine was isolated with the purity suitable for using as reference standard for quality control of herbs and hippadine-containing preparations.