DETERMINATION OF THE TOTAL PHENOLIC CONTENT FROM THE BULBS OF CRINIUM LATIFOLIUM (L.) BY FOLIN-CIOCALTEU METHOD

Abstract

Crinum latifolium is a widely used traditional herb in Viet Nam. Phytochemical study of C. latifolium (L.) bulbs revealed the presence of triterpenoid, alkaloid, polyphenol, saponin, and polyuronid. Polyphenols are phytochemicals, compounds found abundantly in natural plant food sources that have antioxidant properties. The aim of this present study was to develop and validate an analytical method to quantitate the content of total polyphenols (TP) in an extract isolated from C. latifolium (L.) bulbs using UV/Vis spectrophotometric method. The optimum conditions such as analysis time, wavelength and ratio of reagents were examined and identified. Under these conditions, the analytical procedure validation proved the method to be linear, specific, precise, accurate, and reproducible. The total phenolic content of ethanolic extract was 475,58 mg GAE/100 g dry weight.