DETERMINATION OF PAEONIFLORIN FROM RADIX PAEONIAE RUBRAE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

Radix Paeoniae rubrae is a medicinal herb used in traditional medicine as well as in the manufacture of medicinal products for the treatment of chest pain, anti-inflammatory, anti-coagulant, vasodilatation However, the quality of Radix Paeoniae rubrae is suspected because of not tight controlled. The main chemical component of this medicinal herb is paeoniflorin, a monoterpen glycoside. We conducted this study with the aim of developing the quantitative procedures of paeoniflorin in Radix Paeoniae rubrae by HPLC, which can detect cases of counterfeit or exhausted pharmaceuticals, this will help improve the quality control of
this medicinal plant.

The optimized conditions included isocratic mobile phase of 18% of acetonitrile and 82% of phosphate buffer 0.05M pH 4.5. Chromatographic system used Phenomenex Lunar C18 column (250 x 4.6 mm; 5 µm) at 250C, injection volume was 20 µL, flow rate at 1.0 mL/minute, as detector a spectrophotometer set at 230 nm. The procedures enables determination of paeoniflorin in Radix Paeoniae rubrae within 22 minutes. The  analytical procedures was validated the suitability of the system, specificity, linearity (ˆ y = 26.5702x, R = 0.9999), repeatability with RSD =  0.8%, intermediate precision, accuracy with recovery rate 96.6 - 103.7%
and range 6.4 - 25.6 µg/mL.