DETERMINATION OF PROPAFENONE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

A rapid and specific HPLC method has been developed and validated for the determination of propafenone, an antiarrhythmic agent.
Separation was developed on a reverse-phase C18 column (250 mm x 4.6 mm, i.d. 5m) using an acetonitrile-phosphate buffer at a flow rate of 1
ml/min and UV detection of 210 nm. The calibration curves were linear (r2 > 0.999) in the concentration range of 10-100 g/ml. The overall intra- and inter-day variations (RSDs) of the analyte were less than 2% with the recovery ranged within 98102%.